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An adverb clause beginning with "although" requires a comma at its end when it comes at the beginning of the sentence.
The clause coming after the main clause does not require a comma.
Although we work hard, we can not pass the exam.
We can not pass the exam although we work hard.
One note: An adverb clause at the end of the sentence should not have a comma, except -- except -- a clause of contrast, with "although" or "even though" or "though," MAY have a comma to separate it from the main clause if the speaker wants to indicate a pause.