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Reply to "what: subject or object"

Thank you, Gustavo. 

How can I differentiate between what as a subject and what as a subject complement?

They are used with the same word "what".

Let me analyze the two question and tell me if I am right.

1. What is the matter with you?

What: subject

is: to be

the matter: subject  complement

with: preposition

you: object

2. What is your hobby?

What: subject complement

is: to be

your hobby: subject

Thank you so much.