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Hello, Sree, and welcome to the Grammar Exchange.

Yes, there is a difference in meaning between "Do/Don't you go?" and "Do/Don't you go today?" You shouldn't use a comma before "today."

"Do/Don't you go?" means "Are you in the habit of going?," though it is possible for the sentence to have at least other meanings special contexts.

"Do/Don't you go today?" means "Are you scheduled to go today?" or "Is it arranged that you will go today?"

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